Exo-antigens from Paracoccidioides Lutzii Applied to Immunodiagnosis of Paracoccidioidomycosis: an Report of Our Experience in Brazil's Central-west

Beschreibung

In central-west region of Brazil, where Paracoccidioides lutziipredominates, has been described low reactivity in immunological tests usingParacoccidioidesstandard antigen (AgB-339). Here, we aimed to evaluate the performance of exo-antigens fromP. lutziiagainst patient sera from Brazilian central-west region (CWR), and to speculate their influence under PCM immunological diagnose, as well sorological follow-up of these patients. The cell-free culture antigens, known as exo-antigens, were obtained fromP. lutzii(ATCC MYA-826), defined as PlAg, and proved by double agar-gel immunodiffusion (DID) test against 53 PCM suspect serum samples from CWR. The PCM confirmation was given in 81.13% of cases (43/53) by direct exam, biopsy specimens or culture. (Beschreibung aus externen Buchdaten)